Various lysates were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of PTEN antibody (22034-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTEN transfected HEK-293T cells.

IP result of anti-PTEN (IP:22034-1-AP, 4ug; Detection:22034-1-AP 1:500) with DU 145 cells lysate 1800 ug.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PTEN (22034-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

Various lysates were subjected to SDS PAGE followed by western blot with 60300-1-Ig (PTEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60300-1-Ig (PTEN antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60300-1-Ig (PTEN antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PTEN antibody (60300-1-Ig, Clone: 5C10B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of PTEN antibody (10047-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTEN transfected HEK-293T cells.

IP result of anti-PTEN (IP:10047-1-AP, 4ug; Detection:10047-1-AP 1:3000) with HEK-293T cells lysate 2040 ug.

Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29246-1-AP (Phospho-PTEN (Thr382/383) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Cardinal Red™ Anti-Human PTEN (CR-22034) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-60300 (PTEN antibody) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human PTEN (CL488-22034) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PTEN (CL647-22034) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).