MCF-7 cells were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

IP Result of anti-p70S6K (IP:14485-1-AP, 3ug; Detection:14485-1-AP 1:1000) with HeLa cells lysate 3000ug.

Immunofluorescent analysis of HepG2 cells using 14485-1-AP (p70S6K antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

1X10^6 HepG2 cells were stained with 0.2ug p70S6K antibody (14485-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Various lysates were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

WB result of p70(S6K) antibody (14485-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p70(S6K) transfected MCF-7 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14485-1-AP (p70(S6K) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p70(S6K) antibody (14485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 66638-1-Ig (p70(S6K) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66638-1-Ig (p70(S6K) antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66638-1-Ig (p70(S6K) antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 40x lens).

MCF-7 cells were subjected to SDS PAGE followed by western blot with 26587-1-AP (p70(S6K) Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

IP Result of anti-p70(S6K) (IP:26587-1-AP, 4ug; Detection:26587-1-AP 1:300) with A549 cells lysate 1600ug.

Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26587-1-AP (p70(S6K) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human p70(S6K) (CL488-14485) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human p70(S6K) (CL647-14485) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 66638-1-Ig (p70(S6K) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66638-1-Ig (p70(S6K) antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66638-1-Ig (p70(S6K) antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 28735-1-AP (Phospho-p70(S6K) (Thr389) antibody) at dilution of 1:8000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.