Alpha-2-Macroglobulin Polyclonal antibody

Alpha-2-Macroglobulin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13545-1-AP

Print datasheet

Synonyms

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M



经过测试的应用

Positive WB detected inhuman blood tissue, human brain tissue, HepG2 cells, human plasma tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy A2M Ready-To-Use IHC Kit. A2M primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13545-1-AP targets Alpha-2-Macroglobulin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, Plasmodium falciparum
Immunogen Alpha-2-Macroglobulin fusion protein Ag4489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alpha-2-macroglobulin
Synonyms A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M
Calculated Molecular Weight 1474 aa, 163 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC040071
Gene Symbol A2M
Gene ID (NCBI) 2
RRIDAB_10642837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

实验方案

Product Specific Protocols
WB protocol for Alpha-2-Macroglobulin antibody 13545-1-APDownload protocol
IHC protocol for Alpha-2-Macroglobulin antibody 13545-1-APDownload protocol
IF protocol for Alpha-2-Macroglobulin antibody 13545-1-APDownload protocol
IP protocol for Alpha-2-Macroglobulin antibody 13545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Biometal Dyshomeostasis in Olfactory Mucosa of Alzheimer's Disease Patients.

Authors - Riikka Lampinen
Plasmodium falciparumWB

Malar J

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Authors - Takahiro Tougan