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  • KD/KO Validated

AARS2 Polyclonal antibody

AARS2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 22696-1-AP

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Synonyms

AARS2, AARSL, Alanine tRNA ligase, Alanyl tRNA synthetase like, AlaRS, KIAA1270, MT ALARS, MTALARS



经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22696-1-AP targets AARS2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen AARS2 fusion protein Ag18539 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alanyl-tRNA synthetase 2, mitochondrial (putative)
Synonyms AARS2, AARSL, Alanine tRNA ligase, Alanyl tRNA synthetase like, AlaRS, KIAA1270, MT ALARS, MTALARS
Calculated Molecular Weight 985 aa, 107 kDa
Observed Molecular Weight 105-115 kDa
GenBank Accession NumberBC131728
Gene Symbol AARS2
Gene ID (NCBI) 57505
RRIDAB_11182926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JTZ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AARS2(Alanine--tRNA ligase, mitochondrial) is also named as KIAA1270, AARSL, bA444E17.1, AlaRS, AARSL, COXPD8, MTALARS, MT-ALARS and belongs to the class-II aminoacyl-tRNA synthetase family. It catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). It also edits incorrectly charged tRNA(Ala) via its editing domain. The full length 107 kDa protein has a transit peptide with 23 amino acids.

实验方案

Product Specific Protocols
WB protocol for AARS2 antibody 22696-1-APDownload protocol
IHC protocol for AARS2 antibody 22696-1-APDownload protocol
IP protocol for AARS2 antibody 22696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
  • KO Validated
ratWB

Cell Stem Cell

A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.

Authors - Jianyong Du
humanWB

J Hematol Oncol

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Authors - Ning Xu
humanWB

Cancer Med

AARS2 as a novel biomarker for prognosis and its molecular characterization in pan-cancer

Authors - Long Liu