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  • Featured Product
  • KD/KO Validated

ABCD1 Polyclonal antibody

ABCD1 Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 18138-1-AP

Print datasheet

Synonyms

ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN

验证数据展示

  • WB analysis using 18138-1-AP

    WB analysis using 18138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18138-1-AP

    WB analysis of HeLa using 18138-1-AP

    WB result of ABCD1 antibody (18138-1-AP, 1:600) with si-Control and si-ABCD1 transfected HeLa cells..

  • IP experiment of HeLa using 18138-1-AP

    IP experiment of HeLa using 18138-1-AP

    IP result of anti-ABCD1 (IP:18138-1-AP, 4ug; Detection:18138-1-AP 1:700) with HeLa cells lysate 3000 ug.

  • WB analysis of HepG2 using 18138-1-AP

    WB analysis of HepG2 using 18138-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 18138-1-AP

    FC experiment of HeLa using 18138-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ABCD1 (18138-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18138-1-AP targets ABCD1 in WB, IP, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IP, WB, ELISA
Cited Applications WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen ABCD1 fusion protein Ag12757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family D (ALD), member 1
Synonyms ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN
Calculated Molecular Weight 745 aa, 83 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC025358
Gene Symbol ABCD1
Gene ID (NCBI) 215
RRIDAB_2273333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP33897
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

实验方案

Product Specific Protocols
WB protocol for ABCD1 antibody 18138-1-APDownload protocol
IP protocol for ABCD1 antibody 18138-1-APDownload protocol
FC protocol for ABCD1 antibody 18138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated
View Article
humanWB

Signal Transduct Target Ther

Structural insights into substrate recognition and translocation of human peroxisomal ABC transporter ALDP

Authors - Chao Xiong
View Article