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ABLIM2 Polyclonal antibody

ABLIM2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 22433-1-AP

Print datasheet

Synonyms

abLIM 2, ABLIM2, Actin binding LIM protein 2, KIAA1808

验证数据展示

  • WB analysis of mouse brain using 22433-1-AP

    WB analysis of mouse brain using 22433-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22433-1-AP (ABLIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 22433-1-AP

    IHC staining of mouse skeletal muscle using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22433-1-AP (ABLIM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 22433-1-AP

    IP experiment of mouse brain using 22433-1-AP

    IP result of anti-ABLIM2 (IP:22433-1-AP, 4ug; Detection:22433-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

  • IHC staining of human skeletal muscle using 22433-1-AP

    IHC staining of human skeletal muscle using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22433-1-AP (ABLIM2 Antibody) at dilution of 1:50.

  • IHC staining of mouse skeletal muscle using 22433-1-AP

    IHC staining of mouse skeletal muscle using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22433-1-AP (ABLIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22433-1-AP targets ABLIM2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen ABLIM2 fusion protein Ag18198 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin binding LIM protein family, member 2
Synonyms abLIM 2, ABLIM2, Actin binding LIM protein 2, KIAA1808
Calculated Molecular Weight 611 aa, 68 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC122567
Gene Symbol ABLIM2
Gene ID (NCBI) 84448
RRIDAB_11232422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6H8Q1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABLIM2 antibody 22433-1-APDownload protocol
IHC protocol for ABLIM2 antibody 22433-1-APDownload protocol
IP protocol for ABLIM2 antibody 22433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols