• Featured Product
  • KD/KO Validated

ACADS Polyclonal antibody

ACADS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16623-1-AP

Print datasheet

Synonyms

ACAD3, ACADS, Butyryl CoA dehydrogenase, SCAD



经过测试的应用

Positive WB detected inmouse kidney tissue, COS-7 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 16623-1-AP is ACADS Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IF, IP, WB
Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat
Immunogen ACADS fusion protein Ag9922 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
Synonyms ACAD3, ACADS, Butyryl CoA dehydrogenase, SCAD
Calculated Molecular Weight 412 aa, 44 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC025963
Gene Symbol ACADS
Gene ID (NCBI) 35
RRIDAB_10666165
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16219
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.

实验方案

Product Specific Protocols
WB protocol for ACADS antibody 16623-1-APDownload protocol
IHC protocol for ACADS antibody 16623-1-APDownload protocol
IP protocol for ACADS antibody 16623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
mouseWB

Cell Metab

Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity

Authors - Zilong Zhang
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang
humanWB

Cell Rep

HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.

Authors - De Huang
humanWB

Aging (Albany NY)

ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.

Authors - Diyu Chen
  • KD Validated
mouse, ratWB

Arch Toxicol

Protective effects of dioscin against alcohol-induced liver injury.

Authors - Xu Tingting T