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ACAT2 Polyclonal antibody
ACAT2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Caco-2 cells, HepG2 cells, K-562 cells, MOLT-4 cells, mouse liver, rat liver |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver tissue, human lung tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 6 publications below |
IHC | See 2 publications below |
产品信息
14755-1-AP targets ACAT2 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IHC, IP, WB, ELISA |
Cited Applications | IHC, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | ACAT2 fusion protein Ag6503 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | acetyl-Coenzyme A acetyltransferase 2 |
Synonyms | ACAT2, ACTL |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 40-42 kDa |
GenBank Accession Number | BC000408 |
Gene Symbol | ACAT2 |
Gene ID (NCBI) | 39 |
RRID | AB_2220236 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BWD1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for ACAT2 antibody 14755-1-AP | Download protocol |
IHC protocol for ACAT2 antibody 14755-1-AP | Download protocol |
IP protocol for ACAT2 antibody 14755-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol. | ||
Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. | ||
Cancer Cell Int Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer | ||
iScience Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice. | ||
J Agric Food Chem Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice. | ||
Food Res Int Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo. |