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ACAT2 Monoclonal antibody

ACAT2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F5F11

Cat No : 68005-1-Ig

Print datasheet

Synonyms

ACAT2, ACTL

验证数据展示

  • WB analysis using 68005-1-Ig

    WB analysis using 68005-1-Ig

    Various cell lysates were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 68005-1-Ig

    IF Staining of HepG2 using 68005-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAT2 antibody (68005-1-Ig, Clone: 1F5F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HepG2 using 68005-1-Ig

    WB analysis of HepG2 using 68005-1-Ig

    WB result of ACAT2 antibody (68005-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells.

经过测试的应用

Positive WB detected inHepG2 cells, HCT 116 cells, rat brain tissue, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue, rabbit brain tissue
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68005-1-Ig targets ACAT2 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat, rabbit
Immunogen ACAT2 fusion protein Ag6596 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name acetyl-Coenzyme A acetyltransferase 2
Synonyms ACAT2, ACTL
Calculated Molecular Weight 41 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC000408
Gene Symbol ACAT2
Gene ID (NCBI) 39
RRIDAB_2918752
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BWD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACAT2 antibody 68005-1-IgDownload protocol
IF protocol for ACAT2 antibody 68005-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols