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ACOT11 Polyclonal antibody

ACOT11 Polyclonal Antibody for WB,IF/ICC,IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,IF/ICC,IHC,ELISA

Conjugate

Unconjugated

Cat No : 10776-1-AP

Print datasheet

Synonyms

ACOT11, acyl CoA thioesterase 11, BFIT, BFIT1, BFIT2, KIAA0707, STARD14, THEA, THEM1

验证数据展示

  • IF Staining of A431 using 10776-1-AP

    IF Staining of A431 using 10776-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10776-1-AP (ACOT11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human prostate cancer using 10776-1-AP

    IHC staining of human prostate cancer using 10776-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10776-1-AP (ACOT11 antibody) at dilution of 1:100 (under 10x lens).

  • WB analysis using 10776-1-AP

    WB analysis using 10776-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10776-1-AP (ACOT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inDU 145 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10776-1-AP targets ACOT11 in WB,IF,IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,IF/ICC,IHC,ELISA
Tested Reactivity human, mouse, rat
Immunogen ACOT11 fusion protein Ag1176 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-CoA thioesterase 11
Synonyms ACOT11, acyl CoA thioesterase 11, BFIT, BFIT1, BFIT2, KIAA0707, STARD14, THEA, THEM1
Calculated Molecular Weight68.4 kDa
Observed Molecular Weight68-70 kDa
GenBank Accession NumberBC001517
Gene Symbol ACOT11
Gene ID (NCBI) 26027
RRIDAB_2257798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXI4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACOT11(Acyl-coenzyme A thioesterase 11) is also named as BFIT, KIAA0707, THEA.BFIT is mapped to syntenic regions of chromosomes 1 (human) and 4 (mouse) associated with body fatness and diet-induced obesity, potentially linking a deficit of BFIT activity with exacerbation of these traits and supports the transition of this tissue towards increased metabolic activity, probably through alteration of intracellular fatty acyl-CoA concentration(PMID:11696000).

实验方案

Product Specific Protocols
WB protocol for ACOT11 antibody 10776-1-APDownload protocol
IHC protocol for ACOT11 antibody 10776-1-APDownload protocol
IF protocol for ACOT11 antibody 10776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols