ACRV1 Polyclonal antibody

ACRV1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 14040-1-AP

Print datasheet

Synonyms

Acrosomal protein SP 10, acrosomal vesicle protein 1, ACRV1, D11S4365, SP 10, SPACA2



经过测试的应用

Positive WB detected inDU 145 cells, LNCaP cells, PC-3 cells
Positive IP detected inDU 145 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14040-1-AP targets ACRV1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC
Tested Reactivity human
Cited Reactivitymouse, rat
Immunogen ACRV1 fusion protein Ag5109 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acrosomal vesicle protein 1
Synonyms Acrosomal protein SP 10, acrosomal vesicle protein 1, ACRV1, D11S4365, SP 10, SPACA2
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 13-36 kDa
GenBank Accession NumberBC014588
Gene Symbol ACRV1
Gene ID (NCBI) 56
RRIDAB_10640426
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26436
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein ACRV1, also known as SP-10, is an acrosomal matrix protein, which is evolutionarily conserved among mammals. It is associated with the matrix of the acrosomal vesicle in developing spermatids and the acrosomal matrix and membranes of ejaculated sperm (PMID: 8288254). Human ACRV1 protein has 11 isoforms, and molecular weight ranges from 9 to 28 kDa. ACRV1 was predominantly located in round and elongated spermatids in the testis, indicating that ACRV1 may play an important role in mammalian spermatogenesis and may be a target of a contraceptive vaccine (PMID: 21488928; 8874713).

实验方案

Product Specific Protocols
WB protocol for ACRV1 antibody 14040-1-APDownload protocol
IHC protocol for ACRV1 antibody 14040-1-APDownload protocol
IF protocol for ACRV1 antibody 14040-1-APDownload protocol
IP protocol for ACRV1 antibody 14040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice.

Authors - Deqiang Ding
mouseIHC

Int J Mol Sci

BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis.

Authors - Chao Huang
ratIF

Syst Biol Reprod Med

Recellularized rat testis scaffolds with embryoid bodies cells: a promising approach for tissue engineering

Authors - Vitória Frias Batista
mouseIF

Bioinformatics

SATINN: an automated neural network-based classification of testicular sections allows for high-throughput histopathology of mouse mutants

Authors - Ran Yang
mouseIF

Nat Commun

SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility

Authors - Jiali Liu
mouseIF

Arch Toxicol

Extended exposure to tetrabromobisphenol A-bis(2,3-dibromopropyl ether) leads to subfertility in male mice at the late reproductive age

Authors - Yuan-Yuan Li