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ACSM5 Monoclonal antibody

ACSM5 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

2D5D6

Cat No : 67334-1-Ig

Print datasheet

Synonyms

ACSM5, FLJ20581, MACS3

验证数据展示

  • WB analysis of 4T1 using 67334-1-Ig

    WB analysis of 4T1 using 67334-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 67334-1-Ig

    WB analysis of rat liver using 67334-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67334-1-Ig

    IHC staining of human liver cancer using 67334-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67334-1-Ig

    IHC staining of human liver cancer using 67334-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected in4T1 cells, MDA-MB-231 cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67334-1-Ig targets ACSM5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IHC, WB,ELISA
Cited Applications WB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Immunogen ACSM5 fusion protein Ag10056 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name acyl-CoA synthetase medium-chain family member 5
Synonyms ACSM5, FLJ20581, MACS3
Calculated Molecular Weight 208 aa, 23 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC016703
Gene Symbol ACSM5
Gene ID (NCBI) 54988
RRIDAB_2882592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6NUN0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ACSM5 antibody 67334-1-IgDownload protocol
IHC protocol for ACSM5 antibody 67334-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Arthritis Res Ther

Integrative analysis of genome-wide DNA methylation and single-nucleotide polymorphism identified ACSM5 as a suppressor of lumbar ligamentum flavum hypertrophy.

Authors - Yanlin Cao
View Article