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ADARB1 Polyclonal antibody

ADARB1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 22248-1-AP

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Synonyms

ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1



经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22248-1-AP targets ADARB1 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IP, WB, ELISA
Cited Applications IF, IHC, RIP, WB
Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Immunogen ADARB1 fusion protein Ag17743 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)
Synonyms ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1
Calculated Molecular Weight 741 aa, 81 kDa
Observed Molecular Weight 74-86 kDa
GenBank Accession NumberBC065545
Gene Symbol ADARB1
Gene ID (NCBI) 104
RRIDAB_2879047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78563
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADARB1(Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).

实验方案

Product Specific Protocols
WB protocol for ADARB1 antibody 22248-1-APDownload protocol
IP protocol for ADARB1 antibody 22248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.

Authors - Shangming Tang
humanWB,RIP

Basic Res Cardiol

Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAs.

Authors - Karoline E Kokot
mouseIF

Sci Rep

Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.

Authors - Alessia Mirra
mouseWB

FASEB J

Lung fibrosis is induced in ADAR2 overexpressing mice via HuR-induced CTGF signaling.

Authors - Ramani Soundararajan
  • KO Validated
humanWB

J Cell Mol Med

Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis.

Authors - Lei Peng
humanWB

Front Pharmacol

Aberrant Expression of ADARB1 Facilitates Temozolomide Chemoresistance and Immune Infiltration in Glioblastoma.

Authors - Can Lu