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Adenosine A1 Receptor Polyclonal antibody

Adenosine A1 Receptor Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20332-1-AP

Print datasheet

Synonyms

adenosine A1 receptor, Adenosine receptor A1, ADORA1, RDC7

验证数据展示

  • WB analysis of mouse eye using 20332-1-AP

    WB analysis of mouse eye using 20332-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20332-1-AP

    IHC staining of mouse brain using 20332-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20332-1-AP

    IHC staining of mouse brain using 20332-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of SH-SY5Y using 20332-1-AP

    FC experiment of SH-SY5Y using 20332-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug Adenosine A1 Receptor antibody (20332-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inmouse eye tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20332-1-AP is Adenosine A1 Receptor Fusion Protein expressed in E. coli.

Tested Applications FC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen Adenosine A1 Receptor fusion protein Ag14092 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenosine A1 receptor
Synonyms adenosine A1 receptor, Adenosine receptor A1, ADORA1, RDC7
Calculated Molecular Weight 326 aa, 37 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC026340
Gene Symbol ADORA1
Gene ID (NCBI) 134
RRIDAB_2878673
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30542
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADORA1, also named as RDC7, is a receptor for adenosine. It is mediated by G proteins which inhibit adenylyl cyclase. Adenosine is an important mediator of ethanol intoxication and exerts some of its effects via ADORA1 in the central nervous system.

实验方案

Product Specific Protocols
WB protocol for Adenosine A1 Receptor antibody 20332-1-APDownload protocol
IHC protocol for Adenosine A1 Receptor antibody 20332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols