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AE2 Polyclonal antibody

AE2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26332-1-AP

Print datasheet

Synonyms

AE 2, AE2, Anion exchange protein 2, Anion exchanger 2, BND3L, EPB3L1, HKB3, MPB3L, NBND3, SLC4A2

验证数据展示

  • WB analysis using 26332-1-AP

    WB analysis using 26332-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26332-1-AP (AE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 26332-1-AP

    IHC staining of human liver cancer using 26332-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 26332-1-AP

    IHC staining of human liver cancer using 26332-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26332-1-AP targets AE2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen AE2 fusion protein Ag23673 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 4, anion exchanger, member 2 (erythrocyte membrane protein band 3-like 1)
Synonyms AE 2, AE2, Anion exchange protein 2, Anion exchanger 2, BND3L, EPB3L1, HKB3, MPB3L, NBND3, SLC4A2
Calculated Molecular Weight 1241 aa, 137 kDa
Observed Molecular Weight 150 and 70 kDa
GenBank Accession NumberBC028601
Gene Symbol SLC4A2
Gene ID (NCBI) 6522
RRIDAB_2880481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04920
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC4A2, also named as AE2, encodes a Cl-/HCO3- exchanger. It has been reported that the reduced expression of SLC4A2 found in the liver and peripheral blood mononuclear cells of patients may be associated with the hypermethylation of SLC4A2 promoter regions, which might contribute to the pathogenesis of primary biliary cholangitis (PBC). 26332-1-AP antibody detects 150 kDa and 70 kDa (XP_006716157, 666aa) protein in SDS-PAGE. (PMID: 23341620, 32039364)

实验方案

Product Specific Protocols
WB protocol for AE2 antibody 26332-1-APDownload protocol
IHC protocol for AE2 antibody 26332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.

Authors - Eunus S Ali
View Article