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AGER Monoclonal antibody

AGER Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat and More (2)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D4G11

Cat No : 66833-1-Ig

Print datasheet

Synonyms

AGER, RAGE

验证数据展示

  • WB analysis of pig lung using 66833-1-Ig

    WB analysis of pig lung using 66833-1-Ig

    pig lung tissue were subjected to SDS PAGE followed by western blot with 66833-1-Ig (AGER antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 66833-1-Ig

    IHC staining of human lung using 66833-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung using 66833-1-Ig

    IHC staining of human lung using 66833-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inpig lung tissue, rat lung tissue
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66833-1-Ig targets AGER in WB, IHC applications and shows reactivity with Human, Pig, Rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Pig, Rat
Cited Reactivityhuman, mouse
Immunogen AGER fusion protein Ag9657 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name advanced glycosylation end product-specific receptor
Synonyms AGER, RAGE
Calculated Molecular Weight 404 aa, 43 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC020669
Gene Symbol AGER
Gene ID (NCBI) 177
RRIDAB_2882176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15109
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.

实验方案

Product Specific Protocols
WB protocol for AGER antibody 66833-1-IgDownload protocol
IHC protocol for AGER antibody 66833-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Signal

Dapagliflozin alleviates advanced glycation end product induced podocyte injury through AMPK/mTOR mediated autophagy pathway

Authors - Lei Yang
View Article
humanWB

Mol Immunol

S100A11 regulates nasal epithelial cell remodeling and inflammation in CRSwNPs via the RAGE-mediated AMPK-STAT3 pathway.

Authors - Chengcheng Liu
View Article