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AK2 Monoclonal antibody

AK2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3H2C10

Cat No : 66127-1-Ig

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Synonyms

adenylate kinase 2, ADK2, AK 2, AK2, ATP AMP transphosphorylase 2



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AK2 Ready-To-Use IHC Kit. AK2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66127-1-Ig targets AK2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications FC, IF, IHC, WB, ELISA
Tested Reactivity human, rat, mouse
Immunogen AK2 fusion protein Ag17911 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name adenylate kinase 2
Synonyms adenylate kinase 2, ADK2, AK 2, AK2, ATP AMP transphosphorylase 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC009405
Gene Symbol AK2
Gene ID (NCBI) 204
RRIDAB_2881526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54819
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for AK2 antibody 66127-1-IgDownload protocol
IHC protocol for AK2 antibody 66127-1-IgDownload protocol
IF protocol for AK2 antibody 66127-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols