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AK2 Monoclonal antibody
AK2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat, mouse
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3H2C10
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells |
Positive IHC detected in | human liver tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, HeLa cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66127-1-Ig targets AK2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Tested Reactivity | human, rat, mouse |
Immunogen | AK2 fusion protein Ag17911 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | adenylate kinase 2 |
Synonyms | adenylate kinase 2, ADK2, AK 2, AK2, ATP AMP transphosphorylase 2 |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 29 kDa |
GenBank Accession Number | BC009405 |
Gene Symbol | AK2 |
Gene ID (NCBI) | 204 |
RRID | AB_2881526 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P54819 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for AK2 antibody 66127-1-Ig | Download protocol |
IHC protocol for AK2 antibody 66127-1-Ig | Download protocol |
IF protocol for AK2 antibody 66127-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |