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AKT2 Polyclonal antibody

AKT2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 17609-1-AP

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Synonyms

AKT2, PKB beta, PKBB, PKBBETA, PRKBB, Protein kinase Akt 2, Protein kinase B beta, RAC BETA, RAC PK beta



经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue, human liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17609-1-AP targets AKT2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen AKT2 fusion protein Ag11770 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-akt murine thymoma viral oncogene homolog 2
Synonyms AKT2, PKB beta, PKBB, PKBBETA, PRKBB, Protein kinase Akt 2, Protein kinase B beta, RAC BETA, RAC PK beta
Calculated Molecular Weight 481 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC063421
Gene Symbol AKT2
Gene ID (NCBI) 208
RRIDAB_2305350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31751
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.

实验方案

Product Specific Protocols
WB protocol for AKT2 antibody 17609-1-APDownload protocol
IHC protocol for AKT2 antibody 17609-1-APDownload protocol
IP protocol for AKT2 antibody 17609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO J

Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.

Authors - Yingjiang Xu
humanWB,IHC

Respir Res

AKT2 drives cancer progression and is negatively modulated by miR-124 in human lung adenocarcinoma.

Authors - Ting Liu
  • KD Validated
humanWB

Oncotarget

Estrogen regulates miRNA expression: implication of estrogen receptor and miR-124/AKT2 in tumor growth and angiogenesis.

Authors - Cheng-Fei Jiang
ratWB

Food Funct

l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways

Authors - Ling Lin
humanWB

Front Cell Dev Biol

miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis

Authors - Dong Pan
humanWB

Front Cell Dev Biol

Regulation of MicroRNA-497-Targeting AKT2 Influences Tumor Growth and Chemoresistance to Cisplatin in Lung Cancer.

Authors - Lin Wang