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AKTIP Polyclonal antibody

AKTIP Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 14860-1-AP

Print datasheet

Synonyms

AKT interacting protein, AKTIP, FT1, FTS, Fused toes protein homolog

验证数据展示

  • WB analysis of HepG2 using 14860-1-AP

    WB analysis of HepG2 using 14860-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14860-1-AP (AKTIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 14860-1-AP

    IP experiment of HepG2 using 14860-1-AP

    IP result of anti-AKTIP (IP:14860-1-AP, 4ug; Detection:14860-1-AP 1:300) with HepG2 cells lysate 1680 ug.

  • IF Staining of SH-SY5Y using 14860-1-AP

    IF Staining of SH-SY5Y using 14860-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14860-1-AP (AKTIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14860-1-AP targets AKTIP in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen AKTIP fusion protein Ag6662 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name AKT interacting protein
Synonyms AKT interacting protein, AKTIP, FT1, FTS, Fused toes protein homolog
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001134
Gene Symbol AKTIP
Gene ID (NCBI) 64400
RRIDAB_2878087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8T0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AKTIP antibody 14860-1-APDownload protocol
IF protocol for AKTIP antibody 14860-1-APDownload protocol
IP protocol for AKTIP antibody 14860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

AKTIP loss is enriched in ERα-positive breast cancer for tumorigenesis and confers endocrine resistance

Authors - Angel S N Ng
  • KD Validated
View Article