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ALDH1A1-specific Polyclonal antibody
ALDH1A1-specific Polyclonal Antibody for FC, IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human liver tissue, mouse liver tissue, human adrenal gland tissue, K-562 cells |
Positive IHC detected in | human liver tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A549 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 22109-1-AP is ALDH1A1-specific Fusion Protein expressed in E. coli.
Tested Applications | FC, IF/ICC, IHC, WB,ELISA |
Cited Applications | WB, IP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | ALDH1A1-specific fusion protein Ag17358 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | aldehyde dehydrogenase 1 family, member A1 |
Synonyms | ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1 |
Calculated Molecular Weight | 501 aa, 55 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC001505 |
Gene Symbol | ALDH1A1 |
Gene ID (NCBI) | 216 |
RRID | AB_10949189 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P00352 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ALDH1A1-specific antibody 22109-1-AP | Download protocol |
IHC protocol for ALDH1A1-specific antibody 22109-1-AP | Download protocol |
IF protocol for ALDH1A1-specific antibody 22109-1-AP | Download protocol |
FC protocol for ALDH1A1-specific antibody 22109-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Discov Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia. | ||
Commun Biol PRMT3 interacts with ALDH1A1 and regulates gene-expression by inhibiting retinoic acid signaling. | ||
Neoplasma Long noncoding RNA ASAP1-IT1 promotes cancer stemness and predicts a poor prognosis in patients with bladder cancer. | ||
Oncol Lett Renal cell carcinoma with synchronous ipsilateral urothelial carcinoma of the renal pelvis. |