CoraLite®594-conjugated ALDH1A1 Monoclonal antibody
ALDH1A1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1A10A2
验证数据展示
经过测试的应用
| Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 60171-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-60171 targets ALDH1A1 in IF applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | ALDH1A1 fusion protein Ag8551 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | aldehyde dehydrogenase 1 family, member A1 |
| Synonyms | ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1 |
| Calculated Molecular Weight | 501 aa, 55 kDa |
| GenBank Accession Number | BC001505 |
| Gene Symbol | ALDH1A1 |
| Gene ID (NCBI) | 216 |
| RRID | AB_2883431 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P00352 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 ALDH1A1 antibody CL594-60171 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |