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ALDH9A1 Monoclonal antibody

ALDH9A1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C5C9

Cat No : 67372-1-Ig

Print datasheet

Synonyms

ALDH4, ALDH7, ALDH9, ALDH9A1, E3, TMABADH

验证数据展示

  • WB analysis of HEK-293 using 67372-1-Ig

    WB analysis of HEK-293 using 67372-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67372-1-Ig

    WB analysis of HepG2 using 67372-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67372-1-Ig

    IHC staining of human breast cancer using 67372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH9A1 antibody included
  • IHC staining of human breast cancer using 67372-1-Ig

    IHC staining of human breast cancer using 67372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67372-1-Ig (ALDH9A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH9A1 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALDH9A1 Ready-To-Use IHC Kit. ALDH9A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67372-1-Ig targets ALDH9A1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen ALDH9A1 fusion protein Ag24493 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aldehyde dehydrogenase 9 family, member A1
Synonyms ALDH4, ALDH7, ALDH9, ALDH9A1, E3, TMABADH
Calculated Molecular Weight 518 aa, 56 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC151140
Gene Symbol ALDH9A1
Gene ID (NCBI) 223
RRIDAB_2882620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49189
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aldehyde dehydrogenase 9 family member A1(ALDH9A1), belongs to the aldehyde dehydrogenase family of proteins. ALDH9A1 has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. ALDH9A1 catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid. ALDH9A1 has 3 isoforms with the molecular mass of 46-56 kDa.

实验方案

Product Specific Protocols
WB protocol for ALDH9A1 antibody 67372-1-IgDownload protocol
IHC protocol for ALDH9A1 antibody 67372-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols