ALDOA Monoclonal antibody

ALDOA Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D6C2

Cat No : 67453-1-Ig

Print datasheet

Synonyms

ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALDOA Ready-To-Use IHC Kit. ALDOA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67453-1-Ig targets ALDOA in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, pig samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat, pig
Cited Reactivityhuman
Immunogen ALDOA fusion protein Ag17952 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aldolase A, fructose-bisphosphate
Synonyms ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase
Calculated Molecular Weight 39 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC016800
Gene Symbol ALDOA
Gene ID (NCBI) 226
RRIDAB_2882687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04075
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

实验方案

Product Specific Protocols
WB protocol for ALDOA antibody 67453-1-IgDownload protocol
IHC protocol for ALDOA antibody 67453-1-IgDownload protocol
IF protocol for ALDOA antibody 67453-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Food Chem

Phosphorylation and acetylation responses of glycolytic enzymes in meat to different chilling rates

Authors - Yuqiang Bai
humanWB

Oncogene

HMGB1/SET/HAT1 complex-mediated SASH1 repression drives glycolysis and metastasis in lung adenocarcinoma

Authors - Fan Kou