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Alix Monoclonal antibody

Alix Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

IF, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H9D9

Cat No : 67715-1-Ig

Print datasheet

Synonyms

AIP1, ALG 2 interacting protein 1, Alix, ALIX; AIP1, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP



经过测试的应用

Positive WB detected inHSC-T6 cells, A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALIX Ready-To-Use IHC Kit. ALIX primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

67715-1-Ig targets Alix in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF, WB, ELISA
Cited Applications WB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Immunogen Alix fusion protein Ag30437 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name programmed cell death 6 interacting protein
Synonyms AIP1, ALG 2 interacting protein 1, Alix, ALIX; AIP1, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP
Calculated Molecular Weight 868 aa, 96 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC020066
Gene Symbol PDCD6IP
Gene ID (NCBI) 10015
RRIDAB_2882905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WUM4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.

实验方案

Product Specific Protocols
WB protocol for Alix antibody 67715-1-IgDownload protocol
IF protocol for Alix antibody 67715-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Biol Lett

Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway

Authors - Zhaoyi Liang
mouse,humanWB

J Thromb Haemost

Cancer-associated fibroblasts promote venous thrombosis through podoplanin/CLEC-2 interaction in podoplanin-negative lung cancer mouse model

Authors - Toshiaki Shirai
humanWB

Psychiatry Clin Neurosci

Human in vivo evidence of reduced astrocyte activation and neuroinflammation in patients with treatment-resistant depression following electroconvulsive therapy

Authors - Shu-Xian Xu
humanWB

J Microsc

A method for extraction of exosomes from breast tumour cells and characterisation by transmission electron microscopy

Authors - Zhiang Liu
humanWB

ACS Biomater Sci Eng

Enhancing Skin Injury Repair: Combined Application of PF-127 Hydrogel and hADSC-Exos Containing miR-148a-3p

Authors - Qiqi Zhang