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AMT Monoclonal antibody

AMT Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

3E7D4

Cat No : 67532-1-Ig

Print datasheet

Synonyms

aminomethyltransferase, AMT, GCE, GCST, GCVT, NKH

验证数据展示

  • WB analysis of pig liver using 67532-1-Ig

    WB analysis of pig liver using 67532-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67532-1-Ig

    IHC staining of human liver cancer using 67532-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inpig liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67532-1-Ig targets AMT in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications IHC, WB,ELISA
Cited Applications WB
Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman
Immunogen AMT fusion protein Ag26644 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aminomethyltransferase
Synonyms aminomethyltransferase, AMT, GCE, GCST, GCVT, NKH
Calculated Molecular Weight 44 kDa
Observed Molecular Weight37-44 kDa
GenBank Accession NumberBC007546
Gene Symbol AMT
Gene ID (NCBI) 275
RRIDAB_2882751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48728
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AMT antibody 67532-1-IgDownload protocol
IHC protocol for AMT antibody 67532-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Multiomic landscape of immune pathogenesis in Kimura's disease

Authors - Xunyao Wu
View Article