ANAPC5 Monoclonal antibody

ANAPC5 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A7G5

Cat No : 67348-1-Ig

Print datasheet

Synonyms

ANAPC5, APC5, Cyclosome subunit 5



经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67348-1-Ig targets ANAPC5 in WB, IHC, IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen ANAPC5 fusion protein Ag7409 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name anaphase promoting complex subunit 5
Synonyms ANAPC5, APC5, Cyclosome subunit 5
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 85 kDa, 72 kDa
GenBank Accession NumberBC001081
Gene Symbol ANAPC5
Gene ID (NCBI) 51433
RRIDAB_2882606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UJX4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANAPC5 antibody 67348-1-IgDownload protocol
IHC protocol for ANAPC5 antibody 67348-1-IgDownload protocol
IF protocol for ANAPC5 antibody 67348-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols