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ANLN Monoclonal antibody

ANLN Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B4A8

Cat No : 66643-1-Ig

Print datasheet

Synonyms

Actin binding protein anillin, anillin, actin binding protein, ANLN, scra, Scraps



经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, rat brain tissue, mouse brain tissue, HEK-293 cells, U-251 cells, PC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

66643-1-Ig targets ANLN in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA
Cited Applications WB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Immunogen ANLN fusion protein Ag19294 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name anillin, actin binding protein
Synonyms Actin binding protein anillin, anillin, actin binding protein, ANLN, scra, Scraps
Calculated Molecular Weight 1124 aa, 124 kDa
Observed Molecular Weight 110 kDa~120 kDa
GenBank Accession NumberBC034692
Gene Symbol ANLN
Gene ID (NCBI) 54443
RRIDAB_2815000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQW6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anillin, an actin-binding protein, is a conserved cleavage furrow component important for cytokinesis. The human anillin protein localizes to the nucleus during interphase, the cortex following nuclear envelope breakdown, and the cleavage furrow during cytokinesis. It interactions with at least two other furrow proteins, actin and the septins through its C-terminal PH domain and N-terminal actin-binding domain. In anillin knockdown cells, the cleavage furrow ingressed but failed to complete the ingression. Anillin has been found to be overexpressed in tumors and may serve as promising marker for pancreatic carcinoma molecular diagnostics as well as a prognostic indicator for non-small cell lung cancers.

实验方案

Product Specific Protocols
WB protocol for ANLN antibody 66643-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Cycle

CDK1-PLK1/SGOL2/ANLN pathway mediating abnormal cell division in cell cycle may be a critical process in hepatocellular carcinoma.

Authors - Ling Li
humanWB

Clin Exp Pharmacol Physiol

PMID: 32198770

Authors - Min Zhang
humanWB

Front Genet

ANLN and KDR Are Jointly Prognostic of Breast Cancer Survival and Can Be Modulated for Triple Negative Breast Cancer Control.

Authors - Xiaofeng Dai
humanWB

Pathol Res Pract

Vasculogenic mimicry-associated novel gene signature predicted prognosis and response to immunotherapy in lung adenocarcinoma

Authors - Lei Zhang