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ANO2 Monoclonal antibody

ANO2 Monoclonal Antibody for IF-P, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

IF-P, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

2F12G1

Cat No : 67638-1-Ig

Print datasheet

Synonyms

ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B

验证数据展示

  • WB analysis using 67638-1-Ig

    WB analysis using 67638-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 67638-1-Ig

    IHC staining of mouse eye using 67638-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 67638-1-Ig

    IF Staining of mouse eye using 67638-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using ANO2 antibody (67638-1-Ig, Clone: 2F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inY79 cells, NCCIT cells, U2OS cells, MDA-MB-231 cells, T-47D cells, HeLa cells
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67638-1-Ig targets ANO2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF-P, IHC, WB,ELISA
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name anoctamin 2
Synonyms ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B
Calculated Molecular Weight 114 kDa
Observed Molecular Weight 114 kDa
GenBank Accession NumberNM_020373
Gene Symbol ANO2
Gene ID (NCBI) 57101
RRIDAB_2882839
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQ90
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.

实验方案

Product Specific Protocols
WB protocol for ANO2 antibody 67638-1-IgDownload protocol
IHC protocol for ANO2 antibody 67638-1-IgDownload protocol
IF protocol for ANO2 antibody 67638-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols