AARE Polyclonal antibody

AARE Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14758-1-AP

Print datasheet

Synonyms

AARE, ACPH, Acyl peptide hydrolase, Acylaminoacyl peptidase, APEH, APH, D3F15S2, D3S48E, DNF15S2, OPH, Oxidized protein hydrolase



经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, human plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14758-1-AP targets AARE in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Immunogen AARE fusion protein Ag6511 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acylaminoacyl-peptide hydrolase
Synonyms AARE, ACPH, Acyl peptide hydrolase, Acylaminoacyl peptidase, APEH, APH, D3F15S2, D3S48E, DNF15S2, OPH, Oxidized protein hydrolase
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 66-80 kDa
GenBank Accession NumberBC000362
Gene Symbol APEH
Gene ID (NCBI) 327
RRIDAB_2057811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13798
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APH, (APEH, acylpeptide hydrolase), which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. A range of molecular masses have been reported for APH by previous investigators, including 80 kD, 75 kD and 69 kD. This antibody can recognize at least 2 isoforms of APH.

实验方案

Product Specific Protocols
WB protocol for AARE antibody 14758-1-APDownload protocol
IHC protocol for AARE antibody 14758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

mSphere

Internalization of Erythrocyte Acylpeptide Hydrolase Is Required for Asexual Replication of Plasmodium falciparum.

Authors - Rubayet Elahi