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- KD/KO Validated
APJ Polyclonal antibody
APJ Polyclonal Antibody for FC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
FC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells |
| Positive FC detected in | SH-SY5Y cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20341-1-AP targets APJ in WB,FC,ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | FC, WB, ELISA |
| Cited Applications | WB,IHC,IF,FC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | APJ fusion protein Ag14138 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | apelin receptor |
| Synonyms | AGTRL1, Angiotensin receptor like 1, apelin receptor, APJ, APJR, APLNR, G protein coupled receptor APJ |
| Calculated Molecular Weight | 380 aa, 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC032688 |
| Gene Symbol | APLNR |
| Gene ID (NCBI) | 187 |
| RRID | AB_2878676 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35414 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
APJ, also named APLNR and AGTRL1, belongs to the G-protein coupled receptor 1 family. It is a receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. APJ is an alternative coreceptor with CD4 for HIV-1 infection. It may be involved in the development of AIDS dementia. APJ is expressed abundantly in the corpus callosum and spinal cord.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APJ antibody 20341-1-AP | Download protocol |
| FC protocol for APJ antibody 20341-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis. | ||
Sci Transl Med Apelin stimulation of the vascular skeletal muscle stem cell niche enhances endogenous repair in dystrophic mice | ||
Oxid Med Cell Longev Apelin/APJ-Manipulated CaMKK/AMPK/GSK3β Signaling Works as an Endogenous Counterinjury Mechanism in Promoting the Vitality of Random-Pattern Skin Flaps. | ||
Free Radic Biol Med Apelin-13 induces mitophagy in bone marrow mesenchymal stem cells to suppress intracellular oxidative stress and ameliorate osteoporosis by activation of AMPK signaling pathway | ||
EBioMedicine Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling. |
