Apolipoprotein AI Polyclonal antibody
Apolipoprotein AI Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
FC, IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human blood tissue, human brain tissue, human plasma, human ileum tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, HeLa cells |
| Positive FC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14427-1-AP targets Apolipoprotein AI in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
| Cited Applications | IF, IHC, IP, WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | Apolipoprotein AI fusion protein Ag5793 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | apolipoprotein A-I |
| Synonyms | Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 26-30 kDa |
| GenBank Accession Number | BC005380 |
| Gene Symbol | APOA1 |
| Gene ID (NCBI) | 335 |
| RRID | AB_2056524 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02647 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Apolipoprotein AI antibody 14427-1-AP | Download protocol |
| IHC protocol for Apolipoprotein AI antibody 14427-1-AP | Download protocol |
| IF protocol for Apolipoprotein AI antibody 14427-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Enterically derived high-density lipoprotein restrains liver injury through the portal vein. | ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
Br J Cancer A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline. | ||
Theranostics Exosome-mediated delivery of inflammation-responsive Il-10 mRNA for controlled atherosclerosis treatment | ||
Br J Pharmacol A novel apoA-I mimetic peptide suppresses atherosclerosis by promoting physiological HDL function in apoE-/- mice. | ||
Am J Obstet Gynecol Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression. |
