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Apolipoprotein A II Polyclonal antibody

Apolipoprotein A II Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 16845-1-AP

Print datasheet

Synonyms

Apo AII, ApoA II, APOA2, apolipoprotein A II, Apolipoprotein A2

验证数据展示

  • WB analysis of human plasma using 16845-1-AP

    WB analysis of human plasma using 16845-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of human plasma using 16845-1-AP

    IP experiment of human plasma using 16845-1-AP

    IP result of anti-Apolipoprotein A II (IP:16845-1-AP, 4ug; Detection:16845-1-AP 1:500) with human plasma lysate 4000 ug.

  • IF Staining of HepG2 using 16845-1-AP

    IF Staining of HepG2 using 16845-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 16845-1-AP

    IHC staining of human liver using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with APOA2 antibody included
  • IHC staining of human liver using 16845-1-AP

    IHC staining of human liver using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with APOA2 antibody included

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOA2 Ready-To-Use IHC Kit. APOA2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

The immunogen of 16845-1-AP is Apolipoprotein A II Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Apolipoprotein A II fusion protein Ag9863 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein A-II
Synonyms Apo AII, ApoA II, APOA2, apolipoprotein A II, Apolipoprotein A2
Calculated Molecular Weight 100 aa, 11 kDa
Observed Molecular Weight 7-9 kDa (monomer), 15 kDa (dimer)
GenBank Accession NumberBC005282
Gene Symbol APOA2
Gene ID (NCBI) 336
RRIDAB_2878324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02652
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

实验方案

Product Specific Protocols
WB protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
IHC protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
IF protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
IP protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Authors - Yanying Wang
View Article
humanWB

Exp Cell Res

Extracellular vesicle-mediated transfer of the lncRNA-TC0101441 promotes endometriosis migration/invasion.

Authors - Jun-Jun Qiu
View Article
human,mouseWB

Int J Mol Sci

Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes

Authors - Zhonghao Li
View Article