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Arfaptin-1 Monoclonal antibody

Arfaptin-1 Monoclonal Antibody for FC, IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC, IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A10B3

Cat No : 67461-1-Ig

Print datasheet

Synonyms

Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521

验证数据展示

  • WB analysis using 67461-1-Ig

    WB analysis using 67461-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 67461-1-Ig

    IHC staining of mouse kidney using 67461-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IHC staining of mouse kidney using 67461-1-Ig

    IHC staining of mouse kidney using 67461-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IF Staining of mouse kidney using 67461-1-Ig

    IF Staining of mouse kidney using 67461-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse kidney using 67461-1-Ig

    IF Staining of mouse kidney using 67461-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 67461-1-Ig

    IF Staining of HeLa using 67461-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of A549 using 67461-1-Ig

    IF Staining of A549 using 67461-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of Jurkat using 67461-1-Ig

    FC experiment of Jurkat using 67461-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human Arfaptin-1 (67461-1-Ig, Clone:2A10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 90% MeOH .

经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, RAW 264.7 cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue, HeLa cells, A549 cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy ARFIP1 Ready-To-Use IHC Kit. ARFIP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67461-1-Ig targets Arfaptin-1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC, IF/ICC,IF-P, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen Arfaptin-1 fusion protein Ag12097 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor interacting protein 1
Synonyms Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521
Calculated Molecular Weight 373 aa, 42 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC103759
Gene Symbol ARFIP1
Gene ID (NCBI) 27236
RRIDAB_2882691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53367
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
IHC protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
IF protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
FC protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols