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ARIH2 Polyclonal antibody
ARIH2 Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HL-60 cells, mouse brain tissue, mouse kidney tissue |
Positive IP detected in | HeLa cells |
Positive IF detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15006-1-AP targets ARIH2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IP, WB, ELISA |
Cited Applications | WB, IP, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | ARIH2 fusion protein Ag6507 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ariadne homolog 2 (Drosophila) |
Synonyms | ARI 2, ARI2, ariadne homolog 2 (Drosophila), ARIH2, Protein ariadne 2 homolog, TRIAD1, Triad1 protein |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 59 kDa |
GenBank Accession Number | BC000422 |
Gene Symbol | ARIH2 |
Gene ID (NCBI) | 10425 |
RRID | AB_2290024 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95376 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ARIH2 antibody 15006-1-AP | Download protocol |
IF protocol for ARIH2 antibody 15006-1-AP | Download protocol |
IP protocol for ARIH2 antibody 15006-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Immunol ARIH2 is essential for embryogenesis, and its hematopoietic deficiency causes lethal activation of the immune system. | ||
Oncogene The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia. | ||
Cell Death Dis ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.
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