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  • Featured Product
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ARPC1B Polyclonal antibody

ARPC1B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 28368-1-AP

Print datasheet

Synonyms

ARC41, Arp2/3 complex 41 kDa subunit, ARPC1B, p40 ARC, p41 ARC

验证数据展示

  • WB analysis of HeLa using 28368-1-AP

    WB analysis of HeLa using 28368-1-AP

    WB result of ARPC1B antibody (28368-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARPC1B transfected HeLa cells.

  • WB analysis using 28368-1-AP

    WB analysis using 28368-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28368-1-AP (ARPC1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 28368-1-AP

    IP experiment of HeLa using 28368-1-AP

    IP result of anti-ARPC1B (IP:28368-1-AP, 4ug; Detection:28368-1-AP 1:1000) with HeLa cells lysate 1600 ug.

  • IHC staining of human colon cancer using 28368-1-AP

    IHC staining of human colon cancer using 28368-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC1B antibody included
  • IHC staining of mouse spleen using 28368-1-AP

    IHC staining of mouse spleen using 28368-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC1B antibody included

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ARPC1B Ready-To-Use IHC Kit. ARPC1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28368-1-AP targets ARPC1B in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Immunogen ARPC1B fusion protein Ag29001 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin related protein 2/3 complex, subunit 1B, 41kDa
Synonyms ARC41, Arp2/3 complex 41 kDa subunit, ARPC1B, p40 ARC, p41 ARC
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC007555
Gene Symbol ARPC1B
Gene ID (NCBI) 10095
RRIDAB_2881125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15143
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARPC1B also known as ARC41, p41-ARC, or p40-ARC, is one of seven subunits of the human Arp2/3 protein complex, that belongs to the SOP2 family. ARPC1B localizes on centrosomes and has a distinct role in centrosomal homeostasis. ARPC1B is both a physiological activator and substrate of Aurora A kinase, and these interactions help to maintain mitotic integrity in mammalian cells. In recent resaeach, ARPC1B is selected as a candidate prediction marker for sensitivity of Choroidal malignant melanomas to radiotherapy.

实验方案

Product Specific Protocols
WB protocol for ARPC1B antibody 28368-1-APDownload protocol
IHC protocol for ARPC1B antibody 28368-1-APDownload protocol
IP protocol for ARPC1B antibody 28368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Front Immunol

The overexpression of actin related protein 2/3 complex subunit 1B(ARPC1B) promotes the ovarian cancer progression via activation of the Wnt/β-catenin signaling pathway

Authors - Junning Huang
View Article