ASF1A Polyclonal antibody

ASF1A Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10784-1-AP

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Synonyms

ASF1A, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10784-1-AP targets ASF1A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB,ELISA
Cited Applications IF, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen ASF1A fusion protein Ag1233 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Synonyms ASF1A, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC010878
Gene Symbol ASF1A
Gene ID (NCBI) 25842
RRIDAB_2243015
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y294
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

实验方案

Product Specific Protocols
WB protocol for ASF1A antibody 10784-1-APDownload protocol
IHC protocol for ASF1A antibody 10784-1-APDownload protocol
IP protocol for ASF1A antibody 10784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IP

J Virol

Varicella-zoster virus immediate-early 63 protein interacts with human antisilencing function 1 protein and alters its ability to bind histones h3.1 and h3.3.

Authors - Ambagala Aruna P AP
mouseWB,IF

Epigenetics Chromatin

Distinct role of histone chaperone Asf1a and Asf1b during fertilization and pre-implantation embryonic development in mice.

Authors - Xuemei Wang
humanIF

PLoS One

HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

Authors - Jiang Wei-Qin WQ