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ASF1A/B Polyclonal antibody

ASF1A/B Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11011-1-AP

Print datasheet

Synonyms

ASF1A/B, ASF1B, CCG1 interacting factor A II, CIA II, hAsf1, hAsf1b, hCIA II, Histone chaperone ASF1B

验证数据展示

  • WB analysis of mouse thymus using 11011-1-AP

    WB analysis of mouse thymus using 11011-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11011-1-AP

    IHC staining of human lymphoma using 11011-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11011-1-AP (ASF1A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASF1B antibody included
  • IF Staining of HeLa using 11011-1-AP

    IF Staining of HeLa using 11011-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11011-1-AP (ASF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse thymus tissue, HeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ASF1B Ready-To-Use IHC Kit. ASF1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11011-1-AP targets ASF1A/B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen ASF1A/B fusion protein Ag1477 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
Synonyms ASF1A/B, ASF1B, CCG1 interacting factor A II, CIA II, hAsf1, hAsf1b, hCIA II, Histone chaperone ASF1B
Calculated Molecular Weight 202 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC010014
Gene Symbol ASF1B
Gene ID (NCBI) 55723
RRIDAB_2059670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody reacts with the ASF1B and ASF1A protein.

实验方案

Product Specific Protocols
WB protocol for ASF1A/B antibody 11011-1-APDownload protocol
IHC protocol for ASF1A/B antibody 11011-1-APDownload protocol
IF protocol for ASF1A/B antibody 11011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols