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  • KD/KO Validated

ASH2L Polyclonal antibody

ASH2L Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 12331-1-AP

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Synonyms

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2



经过测试的应用

Positive WB detected inCOS-7 cells, COLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12331-1-AP targets ASH2L in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications ChIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, monkey
Cited Reactivityhuman, mouse
Immunogen ASH2L fusion protein Ag3008 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ash2 (absent, small, or homeotic)-like (Drosophila)
Synonyms ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2
Calculated Molecular Weight 628 aa, 69 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC015936
Gene Symbol ASH2L
Gene ID (NCBI) 9070
RRIDAB_2059837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBL3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ASH2L antibody 12331-1-APDownload protocol
IHC protocol for ASH2L antibody 12331-1-APDownload protocol
IP protocol for ASH2L antibody 12331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.

Authors - Esteban A Orellana
humanWB

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu
humanWB,IHC,IF,IP,ChIP

Cancer Sci

ASH2L is involved in promotion of endometrial cancer progression via upregulation of PAX2 transcription.

Authors - Kai Zeng
  • KD Validated
mouseWB,IP

Mol Neurobiol

WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling.

Authors - Minhan Ka