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ATG2A Polyclonal antibody
ATG2A Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IHC, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, MCF-7 cells, Raji cells |
Positive IHC detected in | human tonsillitis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23226-1-AP targets ATG2A in WB, IF, IHC, CoIP, ELISA, Peptide array applications and shows reactivity with human samples.
Tested Applications | IHC, WB, ELISA |
Cited Applications | WB, IF, IHC, CoIP, Peptide array |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | ATG2A fusion protein Ag19709 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ATG2 autophagy related 2 homolog A (S. cerevisiae) |
Synonyms | ATG2A, KIAA0404 |
Calculated Molecular Weight | 1938 aa, 213 kDa |
Observed Molecular Weight | 215 kDa |
GenBank Accession Number | BC110650 |
Gene Symbol | ATG2A |
Gene ID (NCBI) | 23130 |
RRID | AB_2879235 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
UNIPROT ID | Q2TAZ0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for ATG2A antibody 23226-1-AP | Download protocol |
IHC protocol for ATG2A antibody 23226-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol ATG9A protects the plasma membrane from programmed and incidental permeabilization.
| ||
Mol Cell ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation | ||
Nat Commun The autophagy protein ATG9A enables lipid mobilization from lipid droplets.
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EMBO Rep A conserved ATG2-GABARAP family interaction is critical for phagophore formation. | ||
Theranostics The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer. |