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  • KD/KO Validated

ATG4B Polyclonal antibody

ATG4B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 15131-1-AP

Print datasheet

Synonyms

APG4B, ATG4B, AUTL1, Autophagin 1, Cysteine protease ATG4B, hAPG4B, KIAA0943



经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15131-1-AP targets ATG4B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ATG4B fusion protein Ag7261 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG4 autophagy related 4 homolog B (S. cerevisiae)
Synonyms APG4B, ATG4B, AUTL1, Autophagin 1, Cysteine protease ATG4B, hAPG4B, KIAA0943
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC000719
Gene Symbol ATG4B
Gene ID (NCBI) 23192
RRIDAB_2064405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4P1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.

实验方案

Product Specific Protocols
WB protocol for ATG4B antibody 15131-1-APDownload protocol
IHC protocol for ATG4B antibody 15131-1-APDownload protocol
IP protocol for ATG4B antibody 15131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IP

Autophagy

ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1

Authors - Weihong Xie
  • KO Validated
ratWB

Autophagy

RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Authors - Luis Muniz-Feliciano
humanWB,IF

Free Radic Biol Med

The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.

Authors - Zhiguo Li
  • KD Validated
humanWB

Cell Biosci

Discovery and mechanism studies of a novel ATG4B inhibitor Ebselen by drug repurposing and its anti-colorectal cancer effects in mice

Authors - Huazhong Xie
  • KO Validated
humanWB

Cancer Lett

HSF1 upregulates ATG4B expression and enhances epirubicin-induced protective autophagy in hepatocellular carcinoma cells.

Authors - Nan Zhang
humanWB

Front Cell Dev Biol

LncRNA CRNDE Promotes ATG4B-Mediated Autophagy and Alleviates the Sensitivity of Sorafenib in Hepatocellular Carcinoma Cells.

Authors - Lingxi Chen
  • KD Validated