• Featured Product
  • KD/KO Validated

ATG4D Polyclonal antibody

ATG4D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 16924-1-AP

Print datasheet

Synonyms

APG4 D, APG4D, ATG4D, AUTL4, Autophagin 4, Cysteine protease ATG4D



经过测试的应用

Positive WB detected inhuman brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16924-1-AP targets ATG4D in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ATG4D fusion protein Ag10307 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG4 autophagy related 4 homolog D (S. cerevisiae)
Synonyms APG4 D, APG4D, ATG4D, AUTL4, Autophagin 4, Cysteine protease ATG4D
Calculated Molecular Weight 474 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC068992
Gene Symbol ATG4D
Gene ID (NCBI) 84971
RRIDAB_2062024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TL0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.

实验方案

Product Specific Protocols
WB protocol for ATG4D antibody 16924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell

MST4 Phosphorylation of ATG4B Regulates Autophagic Activity, Tumorigenicity, and Radioresistance in Glioblastoma.

Authors - Tianzhi Huang
humanWB

Mol Cell

Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine.

Authors - Joanne Durgan
  • KO Validated
humanWB

Cell Rep

Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation

Authors - Rachel Ulferts
  • KD Validated
humanWB

Mol Ther Nucleic Acids

lncRNA RMST Suppressed GBM Cell Mitophagy through Enhancing FUS SUMOylation.

Authors - Changhong Liu
humanWB

Autophagy

Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.

Authors - Alexander Agrotis
  • KD Validated