- Featured Product
- KD/KO Validated
ATG7 Monoclonal antibody
ATG7 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig and More (4)
Applications
FC, IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2B5A10
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, 4T1 cells, Jurkat cells, pig spleen tissue, HepG2 cells, HeLa cells, HEK-293 cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67341-1-Ig targets ATG7 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
Cited Applications | IF, IHC, IP, WB |
Tested Reactivity | Human, Mouse, Rat, Pig |
Cited Reactivity | human, mouse, rat, canine |
Immunogen | ATG7 fusion protein Ag29172 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | ATG7 autophagy related 7 homolog (S. cerevisiae) |
Synonyms | APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7 |
Calculated Molecular Weight | 78 kDa |
Observed Molecular Weight | 68-78 kDa |
GenBank Accession Number | BC000091 |
Gene Symbol | ATG7 |
Gene ID (NCBI) | 10533 |
RRID | AB_2882599 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O95352 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ATG7 antibody 67341-1-Ig | Download protocol |
IHC protocol for ATG7 antibody 67341-1-Ig | Download protocol |
IF protocol for ATG7 antibody 67341-1-Ig | Download protocol |
FC protocol for ATG7 antibody 67341-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancers (Basel) Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators. | ||
J Cell Physiol Cold exposure-induced endoplasmic reticulum stress regulates autophagy through the SIRT2/FoxO1 signaling pathway | ||
Stem Cell Res Ther Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling. | ||
Front Pharmacol Cardamonin Reduces Acetaminophen-Induced Acute Liver Injury in Mice via Activating Autophagy and NFE2L2 Signaling. |