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ATP2C1 Polyclonal antibody

ATP2C1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13310-1-AP

Print datasheet

Synonyms

ATP dependent Ca(2+) pump PMR1, ATP2C1, ATP2C1A, ATPase 2C1, HHD, hSPCA1, KIAA1347, PMR1, PMR1L, SPCA1

验证数据展示

  • WB analysis using 13310-1-AP

    WB analysis using 13310-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 13310-1-AP

    IP experiment of mouse kidney using 13310-1-AP

    IP result of anti-ATP2C1 (IP:13310-1-AP, 4ug; Detection:13310-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  • IF Staining of HeLa using 13310-1-AP

    IF Staining of HeLa using 13310-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13310-1-AP (ATP2C1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 13310-1-AP

    IHC staining of mouse brain using 13310-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13310-1-AP (ATP2C1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13310-1-AP

    IHC staining of mouse brain using 13310-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13310-1-AP (ATP2C1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, human brain tissue, HeLa cells, rat brain tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

13310-1-AP targets ATP2C1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ATP2C1 fusion protein Ag4143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Ca++ transporting, type 2C, member 1
Synonyms ATP dependent Ca(2+) pump PMR1, ATP2C1, ATP2C1A, ATPase 2C1, HHD, hSPCA1, KIAA1347, PMR1, PMR1L, SPCA1
Calculated Molecular Weight 919 aa, 101 kDa
Observed Molecular Weight 95-103 kDa
GenBank Accession NumberBC028139
Gene Symbol ATP2C1
Gene ID (NCBI) 27032
RRIDAB_10640808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP98194
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP2C1, also known as hSPCA1, belongs to the family of P-type cation transport ATPases. ATP2C1 is a Golgi-localized ATPase that mediates Golgi uptake of cytosolic Ca(2+) and Mg(2+) and has a role in regulating Ca(2+) and Mn(2+) cellular content (PMID: 11741891). ATP2C1 is found in most tissues except colon, thymus, spleen, and leukocytes. It is expressed in keratinocytes (PMID: 15831496, 14632183). Defects in ATP2C1 cause Hailey-Hailey disease (HHD)(PMID: 10615129, 28035777).

实验方案

Product Specific Protocols
WB protocol for ATP2C1 antibody 13310-1-APDownload protocol
IHC protocol for ATP2C1 antibody 13310-1-APDownload protocol
IF protocol for ATP2C1 antibody 13310-1-APDownload protocol
IP protocol for ATP2C1 antibody 13310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

A Negative Feedback Loop in Ultraviolet A-Induced Senescence in Human Dermal Fibroblasts Formed by SPCA1 and MAPK.

Authors - Hongfu Xie
View Article
humanWB

Clin Cosmet Investig Dermatol

The Pathogenic Mechanism of the ATP2C1 p.Ala109_Gln120del Mutation in Hailey-Hailey Disease

Authors - Peiyao Li
View Article
mouseWB

BMC Pulm Med

Tetramethylpyrazine ameliorates endotoxin-induced acute lung injury by relieving Golgi stress via the Nrf2/HO-1 signaling pathway

Authors - Shaona Li
View Article