ATP7B-Specific Polyclonal antibody

ATP7B-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 19786-1-AP

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Synonyms

ATP7B, ATP7B-Specific, Copper pump 2, Copper transporting ATPase 2, PWD, WC1, WD, WND



经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19786-1-AP targets ATP7B-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Cu++ transporting, beta polypeptide
Synonyms ATP7B, ATP7B-Specific, Copper pump 2, Copper transporting ATPase 2, PWD, WC1, WD, WND
Calculated Molecular Weight 157 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberNM_000053
Gene Symbol ATP7B
Gene ID (NCBI) 540
RRIDAB_2878607
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35670
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B.

实验方案

Product Specific Protocols
WB protocol for ATP7B-Specific antibody 19786-1-APDownload protocol
IHC protocol for ATP7B-Specific antibody 19786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Comput Struct Biotechnol J

Impact of Cuproptosis-related markers on clinical status, tumor immune microenvironment and immunotherapy in colorectal cancer: A multi-omic analysis

Authors - Yanfei Shao
humanIF

Medicina (Kaunas)

Curcumin Effect on Copper Transport in HepG2 Cells.

Authors - Anita Berzina
ratWB

Ann Med

Dexmedetomidine enables copper homeostasis in cerebral ischemia/reperfusion via ferredoxin 1

Authors - Qingduo Guo
mouseWB

iScience

Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activation

Authors - Jinsong Wei