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ATXN10 Polyclonal antibody

ATXN10 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15693-1-AP

Print datasheet

Synonyms

ataxin 10, ATXN10, Brain protein E46 homolog, E46L, SCA10

验证数据展示

  • WB analysis of human brain using 15693-1-AP

    WB analysis of human brain using 15693-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15693-1-AP

    IHC staining of human ovary tumor using 15693-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human ovary tumor using 15693-1-AP

    IHC staining of human ovary tumor using 15693-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 15693-1-AP

    IF Staining of HeLa using 15693-1-AP

    Immunofluorescent analysis of Hela cells, using ATXN10 antibody 15693-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of A431 using 15693-1-AP

    IP experiment of A431 using 15693-1-AP

    IP result of anti-ATXN10 (IP:15693-1-AP, 4ug; Detection:15693-1-AP 1:1000) with A431 cells lysate 1200ug.

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, human kidney tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15693-1-AP targets ATXN10 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ATXN10 fusion protein Ag8259 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ataxin 10
Synonyms ataxin 10, ATXN10, Brain protein E46 homolog, E46L, SCA10
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC007508
Gene Symbol ATXN10
Gene ID (NCBI) 25814
RRIDAB_2061167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBB4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

实验方案

Product Specific Protocols
WB protocol for ATXN10 antibody 15693-1-APDownload protocol
IHC protocol for ATXN10 antibody 15693-1-APDownload protocol
IF protocol for ATXN10 antibody 15693-1-APDownload protocol
IP protocol for ATXN10 antibody 15693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.

Authors - Sang Liyun L
View Article
humanWB

J Neurochem

Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.

Authors - Robert J Andrew
View Article