• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

p130Cas / BCAR1 Monoclonal antibody

p130Cas / BCAR1 Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

FC, IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D11C10

Cat No : 67215-1-Ig

Print datasheet

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

验证数据展示

  • WB analysis using 67215-1-Ig

    WB analysis using 67215-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67215-1-Ig

    IHC staining of human breast cancer using 67215-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67215-1-Ig

    IHC staining of human breast cancer using 67215-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human breast cancer using 67215-1-Ig

    IF Staining of human breast cancer using 67215-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using p130Cas / BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human breast cancer using 67215-1-Ig

    IF Staining of human breast cancer using 67215-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of A431 using 67215-1-Ig

    FC experiment of A431 using 67215-1-Ig

    1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human p130Cas / BCAR1 (67215-1-Ig, Clone:2D11C10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, MCF-7 cells, T-47D cells, MDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Positive FC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67215-1-Ig targets p130Cas / BCAR1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity Human
Cited Reactivityhuman
Immunogen p130Cas / BCAR1 fusion protein Ag10604 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name breast cancer anti-estrogen resistance 1
Synonyms BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas
Calculated Molecular Weight 870 aa, 93 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2882507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
IHC protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
IF protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
FC protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Genet

LYPD3, a New Biomarker and Therapeutic Target for Acute Myelogenous Leukemia.

Authors - Tingting Hu
View Article
humanIF

Comput Math Methods Med

Protumor Effects of Histone H3-H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses.

Authors - Liyang Wu
View Article