BCHE Polyclonal antibody

BCHE Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23854-1-AP

Print datasheet

Synonyms

Acylcholine acylhydrolase, BCHE, Butyrylcholine esterase, butyrylcholinesterase, CHE1, Choline esterase II, Cholinesterase, E1, Pseudocholinesterase



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23854-1-AP is BCHE Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, WB
Tested Reactivity human
Cited Reactivityhuman, goat
Immunogen BCHE fusion protein Ag20888 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name butyrylcholinesterase
Synonyms Acylcholine acylhydrolase, BCHE, Butyrylcholine esterase, butyrylcholinesterase, CHE1, Choline esterase II, Cholinesterase, E1, Pseudocholinesterase
Calculated Molecular Weight 602 aa, 68 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberBC008396
Gene Symbol BCHE
Gene ID (NCBI) 590
RRIDAB_2879341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP06276
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCHE(butyrylcholinesterase) is an enzyme expressed in most human tissues. It may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease(PMID:11848688). BCHE is also named as CHE1 and belongs to the type-B carboxylesterase/lipase family. This is a glycoprotein with the molecular weight from 68 kDa to 90 kDa.

实验方案

Product Specific Protocols
WB protocol for BCHE antibody 23854-1-APDownload protocol
IHC protocol for BCHE antibody 23854-1-APDownload protocol
IF protocol for BCHE antibody 23854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Biomed Eng

Genome-edited skin epidermal stem cells protect mice from cocaine-seeking behaviour and cocaine overdose.

Authors - Yuanyuan Li
goatWB,IF

Cells

Improving the Efficiency of Precise Genome Editing with CRISPR/Cas9 to Generate Goats Overexpressing Human Butyrylcholinesterase

Authors - Jia-Hao Wang