• Featured Product
  • KD/KO Validated

BMI1 Monoclonal antibody

BMI1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, monkey

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E10A11

Cat No : 66161-1-Ig

Print datasheet

Synonyms

BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51



经过测试的应用

Positive WB detected inPC-12 cells, HT-1080 cells, U2OS cells, U-937 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOS-7 cells
Planning an IHC experiment? We recommend our IHCeasy BMI1 Ready-To-Use IHC Kit. BMI1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66161-1-Ig targets BMI1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, monkey
Cited Reactivityhuman, mouse
Immunogen BMI1 fusion protein Ag21284 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name BMI1 polycomb ring finger oncogene
Synonyms BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51
Calculated Molecular Weight 37 kDa
Observed Molecular Weight37-43 kDa
GenBank Accession NumberBC011652
Gene Symbol BMI1
Gene ID (NCBI) 648
RRIDAB_2881557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35226
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.

实验方案

Product Specific Protocols
WB protocol for BMI1 antibody 66161-1-IgDownload protocol
IHC protocol for BMI1 antibody 66161-1-IgDownload protocol
IF protocol for BMI1 antibody 66161-1-IgDownload protocol
IP protocol for BMI1 antibody 66161-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Dev Cell

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

Authors - Wenxiu Dai
humanWB

Autophagy

Inhibition of BMI1 induces autophagy-mediated necroptosis.

Authors - Anindya Dey
  • KD Validated
mouseWB

Clin Transl Med

Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.

Authors - Haiyun Chen
mouseWB

J Bone Miner Res

Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription

Authors - Qiuyi Wang
humanWB

Aging (Albany NY)

Identification of a mesenchymal-related signature associated with clinical prognosis in glioma.

Authors - Zhengwei Zhang
human,mouseWB,IHC

Mol Cancer Ther

Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.

Authors - Anindya Dey