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BUB3 Polyclonal antibody

BUB3 Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 10123-2-AP

Print datasheet

Synonyms

BUB3, BUB3L, hBUB3

验证数据展示

  • IF Staining of HepG2 using 10123-2-AP

    IF Staining of HepG2 using 10123-2-AP

    Immunofluorescent analysis of HepG2 cells, using BUB3 antibody 10123-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10123-2-AP targets BUB3 in IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, ELISA
Tested Reactivity human, mouse, rat
Immunogen BUB3 fusion protein Ag0167 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name budding uninhibited by benzimidazoles 3 homolog (yeast)
Synonyms BUB3, BUB3L, hBUB3
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC005138
Gene Symbol BUB3
Gene ID (NCBI) 9184
RRIDAB_2066209
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43684
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BUB3 is a conserved component of the mitotic spindle assembly complex and is an essential compent of spindle-assembly checkpoint (SAC) signaling that operates during early embryogenesis. BUB3 null embryos treated with a spindle-depolymerising agent fail to arrest in metaphase and show an increase in mitotic disarray. In mitosis, the SAC prevents anaphase onset until all chromosomes have been attached to the spindle microtubules and aligned correctly at the equatorial metaphase plate. BUB3 is involved in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments in mammalian oocyte meiosis.

实验方案

Product Specific Protocols
IF protocol for BUB3 antibody 10123-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols