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C19orf12 Polyclonal antibody

C19orf12 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27382-1-AP

Print datasheet

Synonyms

C19orf12

验证数据展示

  • WB analysis of U2OS using 27382-1-AP

    WB analysis of U2OS using 27382-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 27382-1-AP (C19orf12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 27382-1-AP

    IHC staining of human stomach cancer using 27382-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27382-1-AP (C19orf12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inU2OS cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:150-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 27382-1-AP is C19orf12 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited Applications WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen C19orf12 fusion protein Ag26555 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 19 open reading frame 12
Synonyms C19orf12
Calculated Molecular Weight 107 aa, 11 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC063518
Gene Symbol C19orf12
Gene ID (NCBI) 83636
RRIDAB_2880858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSK7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C19orf12 antibody 27382-1-APDownload protocol
IHC protocol for C19orf12 antibody 27382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Free Radic Biol Med

C19orf12 ablation causes ferroptosis in mitochondrial membrane protein-associated with neurodegeneration.

Authors - Changjuan Shao
View Article